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complete t cell medium  (Miltenyi Biotec)


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    Miltenyi Biotec complete t cell medium
    Complete T Cell Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+t+cell+medium/TexMACS+Medium/bio_rxiv__64898__2026__03__11__710988-297-27-41
    Average 97 stars, based on 594 article reviews
    complete t cell medium - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Virus:

    Article Title: Micro-hydrogel injectables that deliver effective CAR-T immunotherapy against 3D solid tumor spheroids
    Article Snippet: Beads were removed immediately before the addition of TAG-72 lentiviral CAR construct and transduction occurred over 48 h in media containing IL-2 (Miltenyi Biotec, Germany). .. Removal of the virus via centrifugation ensues, and cells were transferred to a complete T cell medium comprising TexMACS Medium (Miltenyi Biotec, Germany), human AB serum (5% v/v, Sigma Aldrich, USA), human platelet lysate (5% v/v, Cook Regentec, USA) supplemented with human IL-2, IL-7, IL-15 and IL-21 (Miltenyi Biotec, Germany). .. On day 10 post-transduction CAR+ cells were isolated based on GFP expression using MACSQuant Tyto Cell Sorter (Miltenyi Biotec, Germany).

    Centrifugation:

    Article Title: Micro-hydrogel injectables that deliver effective CAR-T immunotherapy against 3D solid tumor spheroids
    Article Snippet: Beads were removed immediately before the addition of TAG-72 lentiviral CAR construct and transduction occurred over 48 h in media containing IL-2 (Miltenyi Biotec, Germany). .. Removal of the virus via centrifugation ensues, and cells were transferred to a complete T cell medium comprising TexMACS Medium (Miltenyi Biotec, Germany), human AB serum (5% v/v, Sigma Aldrich, USA), human platelet lysate (5% v/v, Cook Regentec, USA) supplemented with human IL-2, IL-7, IL-15 and IL-21 (Miltenyi Biotec, Germany). .. On day 10 post-transduction CAR+ cells were isolated based on GFP expression using MACSQuant Tyto Cell Sorter (Miltenyi Biotec, Germany).

    Isolation:

    Article Title: Efficient NK cell transduction with VSV-G-pseudotyped lentiviral vectors
    Article Snippet: Briefly, PBMCs were isolated from fresh buffy coats followed by T cell isolation by positive selection with CD4/CD8 microbeads (Miltenyi Biotec, Germany). .. Next, the isolated T cells were activated by incubating them for 24 hours with TransAct reagent (Miltenyi Biotec, Germany) at a density of 1×10^6 cells/ml in a complete T cell medium (TexMACS medium supplemented with 12 ng/ml of IL-7 and IL-15 (Miltenyi Biotec, Germany) and 100 U/ml of penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Walthan, MA, USA)). ..



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    Image Search Results


    Intracranial tumor transplantation experiment. (A) Survival duration of normal mice, mice subjected to cranial irradiation, mice with solely transplanted tumors, and mice with transplanted tumors following cranial irradiation (n=6). (B) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (C) IHC of CD4, CD8, CD56, PD1, and Ki67 in peritumoral tissue and tumor tissue. a, brain tissue from the normal mouse group; b, brain tissue from the mouse group subjected to radiotherapy; c, peritumoral brain tissue from the mouse group with tumor induction alone; d, peritumoral brain tissue from the mouse group with tumor induction followed by radiotherapy; e, tumor tissue from the mouse group with tumor induction alone; f, tumor tissue from the mouse group with tumor induction followed by radiotherapy. (D) Bar chart of immunohistochemistry (n=6). (E) Survival time of mice with solely transplanted tumors and mice with transplanted tumors following cranial irradiation after receiving anti-PD-1 therapy (n=6). (F) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (G) Kill rate analysis of GL261 glioma cells co-cultured with CD8 + T cells. Scale bars: 40 µm for IHC images. ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; MRI, magnetic resonance imaging; PD-1, programmed cell death protein 1.

    Journal: Translational Cancer Research

    Article Title: Radiation-activated astrocytes promote tumor progression through CXCL12 secretion and induce resistance to anti-PD-1 immunotherapy

    doi: 10.21037/tcr-2025-1-2652

    Figure Lengend Snippet: Intracranial tumor transplantation experiment. (A) Survival duration of normal mice, mice subjected to cranial irradiation, mice with solely transplanted tumors, and mice with transplanted tumors following cranial irradiation (n=6). (B) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (C) IHC of CD4, CD8, CD56, PD1, and Ki67 in peritumoral tissue and tumor tissue. a, brain tissue from the normal mouse group; b, brain tissue from the mouse group subjected to radiotherapy; c, peritumoral brain tissue from the mouse group with tumor induction alone; d, peritumoral brain tissue from the mouse group with tumor induction followed by radiotherapy; e, tumor tissue from the mouse group with tumor induction alone; f, tumor tissue from the mouse group with tumor induction followed by radiotherapy. (D) Bar chart of immunohistochemistry (n=6). (E) Survival time of mice with solely transplanted tumors and mice with transplanted tumors following cranial irradiation after receiving anti-PD-1 therapy (n=6). (F) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (G) Kill rate analysis of GL261 glioma cells co-cultured with CD8 + T cells. Scale bars: 40 µm for IHC images. ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; MRI, magnetic resonance imaging; PD-1, programmed cell death protein 1.

    Article Snippet: First, CD8 + T cells were cultured in CD8 + T cell complete medium (Procell, CM-M380) and then activated using IL-2 and CD3/CD28 antibodies.

    Techniques: Transplantation Assay, Irradiation, Immunohistochemistry, Cell Culture, Magnetic Resonance Imaging

    Fenofibrate (FNF) enhanced T cell-mediated antitumor immunity in bladder cancer. After T24 cells were pretreated with 0, 50, or 100 µM fenofibrate (FNF) for 24 h: A-B ELISA was used to detect the secretion levels of IFN-γ and TNF-α in the culture supernatants; C Pretreated T24 cells were co-cultured with activated T cells to evaluate the effect of FNF on T cell-mediated cytotoxicity against T24 cells; scale bar = 100 μm. ***, p < 0.001

    Journal: BMC Cancer

    Article Title: Mechanisms for fibrate lipid-lowering drugs in enhancing bladder cancer immunotherapy by inhibiting CD276 expression

    doi: 10.1186/s12885-025-14855-w

    Figure Lengend Snippet: Fenofibrate (FNF) enhanced T cell-mediated antitumor immunity in bladder cancer. After T24 cells were pretreated with 0, 50, or 100 µM fenofibrate (FNF) for 24 h: A-B ELISA was used to detect the secretion levels of IFN-γ and TNF-α in the culture supernatants; C Pretreated T24 cells were co-cultured with activated T cells to evaluate the effect of FNF on T cell-mediated cytotoxicity against T24 cells; scale bar = 100 μm. ***, p < 0.001

    Article Snippet: After incubation with 50 μM or 100 μM FNF for 24 h, cell culture supernatants were collected following centrifugation at 150 g for 15 min. IFN-γ (E-EL-H0108c) and TNF-α (E-EL-H0109c) concentrations in T24 cell supernatants were detected using ELISA kits purchased from Wuhan Elabscience Biotechnology Co., Ltd.

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture